This inexpensive technique is adapted to long observations on thin samples or at low magnification. The samples can be observed in fluorescence, in transmission if they are stained, or by creating phase or interference contrast (DIC).

Widefield microscopy is a simple technique that induces little photobleaching compared to confocal microscopy. However, it is less suitable for thick samples and 3D morphology studies because of the blurring due to the superposition of the observation planes on the image. The use of the Apotome module during acquisition reduces this effect.
Images can be deconvoluted after acquisition via Huygens to increase resolution and contrast.

Available instruments:

• Campus CS
  • Inverted microscope Nikon TI2E
  • Fluorescence stereo microscope Nikon SMZ800
• Campus HU
  • Inverted microscope Leica DMi8
  • Upright microscope Zeiss AxioImager 4 slides Fluo/Color Apotome
  • Fluorescence stereo microscope Leica MZ10F
• Campus Pasteur
  • Upright microscope Zeiss AxioImager Apotome
  • Upright microscope Zeiss Axioplan 2 couleur