One of the challenges of optical imaging in biology is to visualize biological structures and organisms in three dimensions under conditions that preserve their three-dimensional integrity. However, the phenomena of scattering and absorption of light, due to the thickness of the sample, appear and are an intrinsic obstacle to the in-depth exploration of these samples. To meet the needs of 3D imaging of biological samples, a large number of techniques of sample transparisation have been developed, some based on organic solvents, others based on aqueous solutions, with different properties and various applications

Light sheet microscopy is based on a simple principle. The light that illuminates the observed biological object is no longer a cylindrical laser beam, but a sheet of light a few micrometers thick. In an original way, this sheet of light is positioned perpendicular to the objective which will detect the fluorescence emitted by the object. Thus, as on a virtual slicer, the displacement of the object in this sheet of light will allow to carry out optical cuts at all the depths of the sample, thus allowing to observe it in its totality.